Figure 1.
A Coomassie Brilliant Blue-stained 12.5% SDS–PAGE gel showing the purification of TcDHOD. Lane 1, homogenate fraction (5 µg); lane 2, cytoplasmic fraction (5 µg); lanes 3, 4, and 5, pooled fractions after DEAE Sepharose Fast Flow (5 µg), Phenyl Sepharose High Performance (1 µg) and TSK gel G3000SW (1 µg) columns, respectively.