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SDS–PAGE monitoring of the overexpression and purification of XC847. Lane M, molecular-weight markers in kDa; lane 1, whole cell lysate before IPTG induction; lane 2, whole cell lysate after IPTG induction; lane 3, soluble fraction after IPTG induction; lane 4, purified XC847 before TEV cleavage; lane 5, purified XC847 after TEV cleavage. The positions of tagged and free XC1739 are also marked.
