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Kinetic properties of the AnsA wild type and mutants.
| Protein | Vmax (nmoles·min−1) | S0.5 (mM) | nH |
|---|---|---|---|
| Wild type | 16.6 | 1.2 | 2.6 |
| R240A | 41.6 | 5.9 | 4.2 |
| T162A | 29.4 | 4.6 | 1.0 |
| D170Q | 70 | 1.2 | 2.8 |
| Q118D | ND | ||
| T14A | ND | ||
| T14V | ND | ||
| T91A | ND | ||
| T91V | ND | ||
| S61Q | ND |
Values from a single representative experiment are shown. ND =None detected.
