Figure 3.

(A and B) Allele loss in AML and MDS patients with monosomy 7. Loss of heterozygosity was determined as described in Materials and Methods. Half-filled circle, loss of heterozygosity; filled circle, retention of both alleles; lines, noninformative. Patients 21 and 22 show apparent retention of all of the 7q31 loci. Patient 20 shows retention of markers centromeric of D7S2418 and shows a break within the D7S2545-D7S2446 interval. (C) Representative autoradiograph revealing allele loss for D7S486, D7S523, D7S2418, D7S1530, and D7S2446 loci. Lanes: 1, short term cultured Ficoll buoyant fraction enriched for T cells by Con A stimulation; and 2, short term cultured leukemic blasts. ∗, deleted alleles.