Figure 6.

IKK-α(S176A) is a dominant negative inhibitor of IL-1 and TNF-induced NF-κB activation. 293/IL-1RI cells were transiently cotransfected with an E-selectin-luciferase reporter gene plasmid and vector control or IKK-α(S176A) expression vector as indicated. Twenty-four hours after transfection, cells were either left untreated, or stimulated for 6 hr with IL-1 (10 ng/ml) or TNF (100 ng/ml) prior to harvest. Luciferase activities were determined and normalized on the basis of β-gal expression. The values shown are averages (±SEM) of duplicate samples for one representative experiment.