Figure 4.

Enhancement of proliferation and triggering of IFN-γ production from TANK 1 by class I MHC ligands for NKAR proteins. (a) TANK 1 were incubated with various concentrations of SEB and with the various irradiated target cells. Proliferation assays (72 h) were performed as described in Materials and Methods. Each data point was measured in triplicate. The range in each measurement was <5% of the mean of the triplicates. In the absence of SEB, the cpm was <200 regardless of the type of B cell or T cell present. One of six representative experiments is shown. (b) TANK 1 were incubated with irradiated target cells for 72 h, and IFN-γ secretion was measured as described in Materials and Methods. No secretion of IFN-γ was detected in the absence of either TANK 1 or the various targets. (c) TANK 1 were incubated for 72 h with irradiated target cells and 0.5 ng/ml SEB. IFN-γ secretion was measured (in parallel to the proliferation assays) as described in Materials and Methods. No secretion of IFN-γ was detected in the absence of either TANK 1 or the various targets.