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. 2001 Jan 15;107(2):181–189. doi: 10.1172/JCI10934

Figure 4.

Figure 4

Insulin and IGF-1 stimulate phosphorylation of IRS-1 on Ser307 in both 3T3-L1 preadipocytes and adipocytes. (a) 3T3-L1 preadipocytes were stimulated with 100 nM insulin for the indicated time. IRS-1 in cell lysates was immunoprecipitated with αIRS-1 and immunoblotted with αpS307. The same blot was stripped and reprobed with αIRS-1. (b) 3T3-L1 preadipocytes and adipocytes were stimulated for 30 minutes with 100 nM insulin (Ins) and 100 ng/ml IGF-1 (IGF), respectively. IRS-1 immunoprecipitates were immunoblotted with αpS307. The same blot was stripped and reprobed with αIRS-1.