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. 2001 Jan 15;107(2):207–215. doi: 10.1172/JCI11109

Figure 4.

Figure 4

CD8 T cells die by HIVenv-CXCR4 infection. (a) Purified CD4 and CD8 T cells were untreated (0) or treated with soluble X4 (LAI) gp120 and assessed for Fas susceptibility (αFas) or caspase-dependent death (Z-VAD). Ten percent of untreated cells, 23% of cells treated with X4 gp120, and 24% of cells treated with Z-VAD and X4 gp120 (P < 0.006) demonstrated changes in FSC compatible with apoptosis in flow cytometry. (b) CD8 T cells were untreated (0) or treated with SDF1α before incubating with X4 (LAI) gp120 and analyzed for caspase-dependent death (Z-VAD) or susceptibility to Fas-mediated apoptosis (αFas). CD8 T cells were simultaneously analyzed using flow cytometry for CXCR4 expression with and without SDF1α treatment (inset). (c) CD8 T cells were cross-linked with anti-CXCR4 12G5 Ab (αCXCR4 Ab) or matched isotype control (IgG) and assessed for Fas susceptibility (αFas) or caspase-dependent death (Z-VAD). (d) CD4 and CD8 T cells were mixed with 293T cells expressing empty vector (vector), HIVJRFL-env (R5env), or HIVHXB2-env (X4env). Cells were assessed for caspase-dependent death (Z-VAD). Transfected 293T cells were lysed and blotted by duotropic anti-gp160 Ab to verify envelope expression (inset).