Table 2.
Correlation between peptide structure and positive selection
| No. | Sequence | TCR contact residues | Antigenicity, μM | Positive selection, μM |
|---|---|---|---|---|
| 1 | ANERADLIAYLKOATK | AYKQAT | 0.05 | 0.006 |
| 2 | AAAAAAAIAYAAAATK | AYAT | 1.0 | 1.0 |
| 3 | AAAAAAAIAYAKAAAK | AYKA | —* | 14.0 |
| 4 | AAAAAAAIAYAAOAAK | AYQA | — | — |
| 5 | AAAAAAAIAAAAAATK | AAT | — | — |
| 6 | AAAAAAAIAAAAAAAK | AA | — | — |
Antigenicity was measured as the concentration of peptide required to stimulate 40% of the maximum proliferative response from the AND T cell line. Positive selection was measured as the concentration of peptide required to induce 20% of CD4 SP cells in RC.
*
No response at 40 μM.