Figure 3.

Numbers of VacA-vacuolated cells in HeLa cells transiently transfected with dominant-negative dynamin-1 or wild-type, and in nontransfected HeLa cells. Results are expressed as percent of vacuolated cells. Values are means ± SE for six independent experiments with triplicate determinations in which more than 100 cells were counted. The numbers of VacA-vacuolated cells in dominant-negative dynamin-1–transfected HeLa cells (lane 2) were significantly lower than in nontransfected HeLa cells (lane 1). In contrast, no significant difference was observed between wild-type dynamin-1–transfected and nontransfected HeLa cells (lanes 3 and 1). ^AP < 0.01; ^Bnot significant.