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VacA internalization by HeLa cells is not affected by dominant-negative dynamin-1. HeLa cells transiently transfected with dominant-negative dynamin-1 were double-stained with anti–dynamin-1 antibody (upper panels) and anti-VacA antibody (lower panels) 1, 3, and 12 hours after VacA intoxication. VacA attached to (1 hour) and internalized into (3 hours and 12 hours) dominant-negative dynamin-1–transfected cells (arrowheads) as well as nontransfected cells. Bar, 10 μm. ×400.
