Table 1.
Quantitative analysis of allograft histology
| Animal | CD3 | CD4 | CD8 | CD45RB | CD25 |
|---|---|---|---|---|---|
| D6: Control | |||||
| 1 | 227 (2.2) | 112 (0.6) | 98 (0.5) | 126 | 12 |
| 2 | 390 (1.2) | 169 (0) | 154 (0.5) | 214 | 18 |
| 3‡ | |||||
| D6: Treated | |||||
| 1 | 238 (0.6)* | 150 (0.2) | 84 (0.4) | 106 | 14 |
| 2 | 310 (0.7) | 234 (0.5) | 84 (0.4) | 112 | 24 |
| 3 | 268 (0) | 146 (0.4) | 47 (0) | 63 | 44 |
| D11: Control | |||||
| 4 | 268 (14.3) | 106 (3.5) | 103 (4.9) | 78 | 19 |
| 5 | 526 (8.2) | 338 (2.7) | 300 (3.2) | 255 | 95 |
| 6‡ | |||||
| D11: Treated | |||||
| 4 | 274 (0)* | 216 (0)* | 78 (0)* | 105 | 4† |
| 5 | 214 (1.6) | 113 (0.3) | 91 (1.3) | 62 | 3 |
| 6 | 295 (1) | 196 (0.4) | 97 (0.4) | 122 | 14 |
Immunoperoxidase positive cells in peri-islet infiltrates from untreated and MB23G2-treated animals 6 and 11 days after transplantation were counted (in 20 squares of a grid). Cells actually invading the islets were counted separately and are presented within the parentheses as the mean number of cells/islet.
*
P ≤ 0.05; treatment vs. control groups on same day.
†
P ≤ 0.05; treated animals on day 11 vs. day 6.
‡
Not quantitated due to severe fibrosis and lack of discernable islets in the area of the allograft.