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Tpl-2 collaborates with NFATp and NFATc but not NFATx to activate IL-2/CAT and NFAT/CAT reporters. Jurkat cells were cotransfected with expression constructs of wild-type Tpl-2 and NFATp, NFATc, or NFATx in combination with IL-2/CAT (A) or NFAT/CAT (B) reporter constructs. All transfections were done in quadruplicate and expression was normalized based on the expression of βgal from a cotransfected CMV-β-Gal construct.
