Figure 6.

Dominant negative mutant Tpl-2K167M inhibits the activation of NFAT by anti-CD3 but not by PMA plus ionomycin. Relative CAT activity in lysates of TAg-Jurkat cells transiently transfected with the NFAT/CAT reporter construct and carboxyl-terminally truncated or kinase-dead (K167M) Tpl-2 expression constructs is shown. Selected cultures were stimulated with the human anti-CD3 antibody OKT3 or PMA and ionomycin as indicated. All transfections were done in quadruplicate. Similar results were obtained in two independent experiments.