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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Exp Gerontol. 2007 Jan 26;42(6):483–497. doi: 10.1016/j.exger.2007.01.004

Fig. 5.

Fig. 5

Northern blots of MnSOD over-expression. Total RNA was isolated from adult male flies containing no MnSOD transgene, and single, and double MnSOD insertions, and loaded at 1X and 2X concentrations as indicated. Rp49 is used as a loading control. (A) Northern blot of MnSOD over-expression using the powerful P{rtTA}M1(3) driver. Endogenous MnSOD transcript is readily detected with longer exposures (data not shown). (B) Doxycycline was used to activate expression of MnSOD at the indicated dilutions using the P{rtTA}E2(3) and P{rtTA}M1(3) drivers.