Table 1.

Prevention of HIV-1-induced suppressor cell generation by anti-IFNα and anti-Tat Abs

Donor	Exp.	Control Abs	T cell proliferation, %
			Antibodies to
			IFNα	Tat	IFNα + Tat
A	1	12	ND	ND	117
	2	25	121	19	104
	3	20	90	14	99
B	1	8	109	8	74
C	1	26	101	63	101
	2	17	12	ND	85
	3	28	26	19	84
D	1	59	94	67	84
	2	37	103	6	86
E	1	16	ND	ND	100
	2	55	ND	ND	88
	3	67	ND	ND	104
F	1	25	93	6	88
	2	17	96	16	83
G	1	26	24	17	106
	2	11	104	87	107
	3	34	114	107	107
H	1	35	ND	ND	87
	2	41	122	31	114
I	1	26	90	60	98
J	1	28	95	51	91
K	1	10	17	7	118
L	1	9	ND	ND	92

PHA-activated PBMC from seronegative subjects were infected by HIV-1 and cultured in the presence of specific (experimental samples) or irrelevant (control samples) Abs. To test suppressor activity in these cultures, SEF-stimulated PBMC were cultured in the presence (test cultures) (T) or the absence (reference cultures) (R) of 6-day cultured cells after their irradiation. T cell proliferation of experimental or control test cultures (T) was compared with that of reference cultures (R), and results are expressed as T:R × 100. In these assays, T cell proliferation of SEB-stimulated PBMC cocultured with uninfected cultured cells was identical to that of reference cultures (100%). ND, not done.