Figure 1.

Production of C-C chemokines by PBMC from seronegative individuals. T cell proliferation was assayed by ³H-thymidine test, and C-C chemokine production was measured by ELISA in culture SN. (a) Kinetics of T cell proliferation (—◊—) and of C-C chemokines secretion: MIP-1α (—•—), MIP-1β (—▪—), and RANTES (—▴—). Only background values of chemokines production (<0.5 ng/ml) and T cell proliferation (<500 cpm) were found for resting cells. A representative experiment is shown. (b) Variations of chemokines production by PBMC from five different seronegative donors. (c) Box plot analysis of C-C chemokine production in high risk HEH. HEH (white boxes) compared with control seronegative donors (black boxes). Box limits correspond to 50% percentile of each group, and the horizontal bar corresponds to the median. Asterisks refer to a statistical difference (P = 0.0063 for MIP-1α, P = 0.0223 for MIP-1β, and P = 0.0361 for RANTES).