Skip to main content
. 1998 Mar 31;95(7):3857–3861. doi: 10.1073/pnas.95.7.3857

Figure 3.

Figure 3

HIV-1 infection of activated PBMC from HEH. HIV-1 infection of PBMC in the presence of recombinant C-C chemokines (MMR) was assessed by p24 ELISA test. (a) Kinetics of HIV-1 infection of seronegative cells by HIV-1 NSI, in the absence (—▪—) or presence (—○—) of 40% of SN or of MMR (—□—). The assay included cells from three control seronegative (a1–a3); 3 HEH without Δ32 abnormality (+/+) (a4–a6); and 3 HEH with Δ32 abnormality (Δ32/+) (a7–a9) (a7 = RIUU; a8 = PRET; and a9 = GHGIO). (b) Kinetics of HIV-1 infection by primary isolate (AUD) in control cells in the absence (—▪—) or presence (—○—) of SN. (c) Dose-dependent inhibition of HIV-1 infection by autologous cell SN. This assay was performed for 7 days on cells from three HEH with Δ32 abnormality (—•—) RIUU; (—▪—) PRET and (—▵—) GHGIO. Cells were infected with either HIV-1 Z96 (c1) or HIV-1 AUD (c2). (d) Effect of a mixture of neutralizing Abs to MMR on HIV-1 infection of PBMC from one HEH subject with Δ32 abnormality (d1 and d2) and one control donor (d3 and d4). Cells were infected with either HIV-1 Z96 (d1 and d3) or HIV-1 AUD (d2 and d4). Absence of SN (—▪—); presence of SN (—○—); and presence of SN preincubated with neutralizing Abs (—x—).