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pH-dependent hyperpolarization-activated currents in cells expressing endogenous levels of ClC-2 (S1) and cells overexpressing ClC-2 (S2) are chloride-selective. Current–voltage (I–V) plots of HACCs from S1 and S2 cells in Cl−, I−, and Glu− with an extracellular pH of 3.8 (n = 3 for each). Error bars indicate the standard error of the mean. Asterisks indicate a significant difference (P < 0.05) from the Cl− I–V plot by anova and Bonferroni tests.
