Figure 5.

Southern analysis of TCR gene rearrangements in leukemias from transgenic mice. (A) Clonal TCR rearrangements. Southern blot analysis of DNA isolated from the spleens or thymuses of leukemic and nonleukemic mice. DNAs were digested with EcoRI. Blots were hybridized with a probe specific for the TCR Jβ₂ gene segment. Lanes: 1, spleen TG25; 2, spleen non-TG littermate; 3, spleen TG28 (partial digest); 4, spleen TG 43; 5, spleen TG 45; 6, spleen TG 47; 7, thymus TG 47; 8, spleen TG 39; and 10, thymus TG39. The strong 2.2-kb bands represent the TCR Jβ₂ gene in its germ-line configuration; arrows point to weaker hybridizing bands indicating the presence of clonal TCR gene rearrangements. (B) Expression of a specific TCR-Vβ on the surface of mouse leukemic cells. Single-cell suspensions of splenocytes from a representative tumor were stained with anti-Vβ6 antibodies (black) or with control antibody (FITC control, gray) followed by fluorescence-activated cell sorter analysis.