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. Author manuscript; available in PMC: 2007 Sep 24.
Published in final edited form as: Biochemistry. 2005 Feb 22;44(7):2632–2641. doi: 10.1021/bi0477900

Figure 7. Effect of ionic strength on 7-EFC dealkylation as a function of CYP1A2 concentration.

Figure 7

Reaction rates for PROD using binary and mixed reconstituted systems were carried out at low (50 mM) and high (300 mM) ionic strength HEPES buffer, using a fixed CYP2B4 (0.05 μM) and reductase (0.025 μM) concentrations, while varying CYP1A2 as described in Methods.