Table 2. Kinetic constants for the simulated curves for 7-Ethoxyfluorocoumarin metabolism.
50 mM HEPES | 300 mM HEPES | |
---|---|---|
Kar | 0.004 | 0.0073 |
kar | 56 | 35 |
Kbr | 0.036 | 0.0177 |
Kbr | 2144 | 1080 |
Kbar | 0.093 | 0.00255 |
Kbar | 434 | 35 |
Krbar** | 0.01 | 0.01 |
krbar* | 2197 | 1115 |
In these simulations, the rate constant for the RBAR complex was set as the sum of the AR and BR complexes. This was done to constrain the simulations under the assumption that the quaternary complex would have a rate constant similar to the sum of the binary complexes.
Under the conditions of this experiment, the value for Krbar could not be determined. Although the data were fit using these parameters, this value was not unique, and could have a range over 4 orders of magnitude without significantly affecting the values of the other rate constants.