Fig. 5.
Steady-state kinetics of dodecanoic acid hydroxylation by CYP52A21. Each point is a mean ± SD of triplicate assays. The estimated parameters were kcat 33 ± 1 nmol of total hydroxylated dodecanoic acid formed/min/nmol of P450, Km 57 ± 2 μM, and kcat/Km 0.58 ± 0.03.