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. 1998 Mar 31;95(7):3914–3919. doi: 10.1073/pnas.95.7.3914

Figure 2.

Figure 2

PAF or IL-1β do not modify TFIID-DNA binding. EMSA using NPXTs derived from HEK cells and the mutant and consensus sequence for the basal transcription factor TFIID. Arrow depicts unique TFIID-shifted complex (34, 45) used for signal quantitation in the bar graph shown below. A 60-fold excess of unlabeled TFIID consensus sequence (“cold” competition assay) almost completely eliminated TFIID gel shift. Rabbit polyclonal antibody specific for human TFIID was used in the gel supershift assay. Exposure time = 36 hr. n = 4; mean ± SD; significance of PAF- or IL-1β-induced TFIID factor over control, P > 0.68.