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. 1998 Mar 31;95(7):3914–3919. doi: 10.1073/pnas.95.7.3914

Figure 3.

Figure 3

Dose-dependent inhibition by BUDeR or DEX of DNA binding by cis-acting transcription factors AP1, GAS, and NF-κB in HEK cells activated by PAF (A) or IL-1β (B). Relative signal intensity of DNA binding activity as determined by EMSA using NPXTs derived from HEK cells and reacted with the target oligonucleotide consensus sequences for the transcription factors AP1, GAS, or NF-κB. Leftmost lanes represent the relative signal intensity of a typical free oligonucleotide with no NPXT. Dashed line represents HEK control NPXT and DNA consensus sequences for transcription factors AP1, GAS, or NF-κB (Table 1) equal to 100. Compared with controls, both PAF (A) and IL-1β (B) elicited a 1.9- to 2.6-fold increase in transcription factor-DNA binding for AP1, GAS, and NF-κB. (M) = molar. n = 4; mean ± SD; significance of induced factors over control: ∗, P < 0.001, ∗∗, P < 0.01, ANOVA. Levels of TFIID-DNA binding remained consistently unchanged (P ≥ 0.68, ANOVA; Figs. 1 and 2).