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. 1998 Mar 31;95(7):3914–3919. doi: 10.1073/pnas.95.7.3914

Figure 4.

Figure 4

PAF or IL-1β, but not 1L-6, induces COX-2 gene expression in HEK cells. Dose-dependent inhibition of this induction by BUDeR or DEX. (A) Diagram of the human COX-2 promoter showing transcription factor DNA binding consensus sequences from −1,000 and +165 bp relative to the start of transcription (+1 bp). DNA binding consensus sequences are described in Tables 1 and 2. Note that the TFIID site doubles as a GAS binding site. (B) Representative gel showing COX-1 and COX-2 RNA message abundance after treatment with PAF (100 nM), IL-1β (10 ng/ml), IL-6 (10 ng/ml), or DEX and BUDeR (each at 10−7 M). (C) RNA message levels quantitated from B. n = 4; mean ± SD; significance of induced RNA levels over control, ∗, P < 0.001, ∗∗, P > 0.05, ANOVA. (D) Dose-dependent inhibition by BUDeR or DEX on PAF- or IL-1β-induced changes on COX-1 and COX-2 RNA message abundance. n = 4; mean ± SD; significance of GC suppression at 10−7 M compared with no treatment, ∗, P < 0.001, ANOVA.