Table 1.
cDNA plasmids
| Plasmid | Segment | Remarks | Ref. or source |
|---|---|---|---|
| pKT230 | Vector for pseudomonads | 16 | |
| pT7T3 19U | amp, PT7, PT3, lacZ; cloning and transcription vector | Pharmacia | |
| pLM656, 4,063-bp transcript | M | amp, PT7; exact copy of segment M in pT7T3 19U | 14 |
| pLM659, 2,948-bp transcript | S | amp, PT7; exact copy of segment S in pT7T3 19U | 28 |
| pLM687, 6,374-bp transcript | L | amp, PT7; copy of segment, L in pT7T3 19U | 29 |
| pLM1053, 1,810-bp transcript | M | pLM656 with a deletion from PmII to PstI | 10 |
| pLM1114, 6,718-bp transcript | MS 5′ M | Segment M cut at PstI and ligated to BamHI site of S | This study |
| pLM1241, 7,215-bp transcript | SM 5′ S | Segment S cut at SacII and ligated to HindIII of M; lacH is in PstI site | This study |
| pLM1417, 798-bp transcript | S 5′S | Segment S to EcoRV ligated to 100-base | This study |
| pLM1809, 13,507-bp transcript | SML 5′S | Segment S to SacII ligated to HindIII of M; PstI of M ligated to SmaI of L, with lacH in PstI site of L | 11 |
| pLM2064, 14,059-bp transcript | SML 5′S | pLM1809 with EcoRV/XbaI piece of M replacing PstI/XbaI | This study |