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. Author manuscript; available in PMC: 2007 Sep 24.
Published in final edited form as: Protein Expr Purif. 2005 Apr 13;43(1):33–43. doi: 10.1016/j.pep.2005.03.023

Figure 1.

Figure 1

Plasmid pME4 was isolated by functional screening of a P. maltophilia DNA library for colonies expressing sarcosine oxidase activity and is missing 6 bp at the 5′ end of soxB. Plasmid pME401 is an intermediate construct that contains the complete soxB and soxD genes and a small portion from the 5′ end of soxA. Plasmid pME410 contains complete genes for all four pTSOX subunits plus an unsequenced region (unseq.) of P. maltophia DNA. Plasmid pME420 is a derivative of pME410, obtained after insertion of a fragment from the 3′ end of the corynebacterial glyA gene. For clarity, the vector-encoded lacZ gene fragment in pME4 is shown at 5 times its actual size. Arrowheads indicate the direction of transcription.