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. Author manuscript; available in PMC: 2007 Sep 24.
Published in final edited form as: Protein Expr Purif. 2005 Apr 13;43(1):33–43. doi: 10.1016/j.pep.2005.03.023

Figure 2.

Figure 2

Plasmid pME430 was obtained by transferring the entire insert from pME420 (see Figure 1) into pBluescript II SK(+). Plasmid pGZ01 is an intermediate construct obtained after transfer of the insert from pME430 into a vector encoding a (His)6 affinity tag. Plasmid pGZ25 is a derivative of pGZ01 where the vector-encoded (His)6 tag is positioned immediately downstream of soxG. For clarity, the vector-encoded lacZ gene fragment in pME430 is shown at 5 times its actual size. Arrowheads indicate the direction of transcription.