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. Author manuscript; available in PMC: 2007 Sep 24.
Published in final edited form as: Protein Expr Purif. 2005 Apr 13;43(1):33–43. doi: 10.1016/j.pep.2005.03.023

Figure 5.

Figure 5

Coomassie blue stained SDS-PAGE gel of the purification of His-tagged recombinant pTSOX. Lane 1 is the soluble cell lysate (10 μg) obtained after growth of E. coli BL21(DE3)/pGZ25 in media containing 50 g sarcosine/L. Lane 2 (10 μg) is after purification on a Co2+ affinity column. Lane 3 (10 μg) and lanes 4 and 5 (15 μg) were obtained after further purification on a Phenyl-Sepharose column. Molecular markers are shown in lane M.