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Fluorescence image of a myotube transfected with an alpha-actinin (N-273) fragment. (a) Alpha-actinin (N-273) fragment localized to the Z-bands of mature myofibrils one day post transfections just like the full construct alpha-actinin. (b) In contrast to transfections with the full construct of alpha-actinin, myotubes expressing the alpha-actinin fragment exhibited increasing numbers of disassembled myofibrils after one or more days.
