Primer	Sequence	Size (bp)	Reference
AQP-0	F: acg gct caa gag tgt ttc tga	189	[63]
	R: tcc cca cag tct ctt tct tca t
AQP-1	F: ctg tgg tgg ctg agt tcc tg	344	[64]
	R: att tcg gcc aag tga gtt ctc
AQP-2	F: atg tgg gaa ctc aga tcc ata gcc ttc tcc	816	[64]
	R: tca ggc ctt gct gcc gcg agg cag gct
AQP-3	F: gag atg ctc cac atc cgc tac	485	[64]
	R: cac aca ata agg gct gct gtg
AQP-4	F: ctc tgc ttt gga ctc agc att g	570	[64]
	R: ttc ctt tag gcg acg ttt gag
AQP-5	F: gcc aca tca atc cag cca tt	383	[64]
	R: aaa gat cgg gct ggg ttg at
AQP-6	F: ctg ctt gta tgg tgt ccc tgg tgt	262	[65]
	R: ggc ctt gga aaa cta act gga tgg
AQP-7	F: atg gcc ggt tct gtg ctg	810	[66]
	R: tct caa gaa ccc tgt ggt gg
AQP-8	F: aag acc atg ctg cta att cc	275	[63]
	R: tcc aca atg aca gag aaa cc
AQP-9	F: atg cct tct gag aag gac gg	888	[67]
	R: cta cat gat gac act gag ct
RT-PCR was performed as described in the Materials and Methods section. As positive controls, total RNA from rat kidney, lung, and liver were used. The size of PCR products for different AQP forms matched the predicted size. The relative intensity of protein bands is indicated as follows: (-) negative, (+) detectable, (++) strong, and (+++) very strong. In glioma cells lines, AQP1 was the predominant form, with lesser amounts of AQP6 and AQP8 also detectable.