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. 2007 Oct 1;21(19):2461–2472. doi: 10.1101/gad.1584907

Figure 3.

Figure 3.

Effects of the mutations on other aspects of OR regulation. Response of PRM.lacZ reporters (A) and PR.lacZ reporters (B,C) to CI (A,C), or Cro (B) supplied by IPTG-inducible plasmids. The λ fragment extends from the +62 of PRM to the +43 of PR. The inset in C shows reporter expression with CI supplied from a λatt80 prophage. (D) Translational fusion reporters showing the effect of the OR mutations on cI translation from PRE mRNA. λ PRE is substituted by the constitutive 186 pB promoter. (A–D) Error bars and ranges are 95% confidence limits.