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. 2007 Oct 1;21(19):2485–2494. doi: 10.1101/gad.1588807

Figure 1.

Figure 1.

Characterizing the tlc1-tm mutant. (A) Telomere lengths of the indicated strains were determined by telomere PCR of telomere VI-R. The size of the PCR product is the sum of the telomere length, the distance of the subtelomeric primer to the beginning to the telomeric tracts, and the size of the primers used in the telomere PCR (59 bp). Telomere lengths were calculated to be 353 bp and 271 bp for TLC1 and tlc1-tm strains, respectively. The calculated telomere lengths are an average of telomere lengths from two different isolates of each strain. (B) Telomere PCR products from A amplified from the tlc1-tm mutant were cloned and sequenced. Three representative sequences are shown. The complete set of sequences is shown in Supplementary Figure 1. Mutant divergent regions of each sequence (in red) were used to derive a mutant consensus sequence of [(TG)0–4TGG)]nATTTGG. Bolded sequences highlight occurrences of GGG trinucleotide incorporation by the mutant telomerase.