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. Author manuscript; available in PMC: 2007 Sep 25.
Published in final edited form as: Biochim Biophys Acta. 2006 Dec 15;1768(4):772–793. doi: 10.1016/j.bbamem.2006.12.002

Fig. 12.

Fig. 12

Stimulation of LARG’s guanine nucleotide exchange activity by G12α depends on Tec kinase, but that of G13α does not. Top: linear diagram of RhoGEF proiesn with relative location of the DHPH GEF domains, the RGSL domain (for extended RGS domain) and, when present, the PDZ domain. Bottom, in vitro stimulation of GDP release from RhoA by recombinant G12α and G13α in the absence and presence of Tec kinase and ATP. Tec was prepared by expressing a myc-tagged Tec cDNA in COS cells and concentrating the expressed protein by immunoprecipitation. G12α, G13α and LARG (without its N-terminal PDZ domain) were purified from Sf9 insect cells infected with the corresponding recombinant baculovirus. For further details see (Suzuki et al., 2003 [111]).