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Induction of apoptosis by anti-Fas antibody, TNFα and C2-ceramide. (A–C) Cells were exposed to TNFα, or anti-Fas antibody alone or in the presence of CHX and assayed for viability. (D) SK-N-SH cells were exposed to C2-ceramide and tested for viability. (E) HeLa cells exposed to C2-ceramide and tested for degradation of DNA. The procedures were as described in Materials and Methods.
