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. 2007 Jun 8;1:16. doi: 10.1186/1752-153X-1-16

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Copyright © 2007 Dalal et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Precipitation of enzymatic activities with organic solvents: Three different precipitants (acetone, dimethoxyethane (DME) and n-propanol, 5 ml each) were added to crude Pectinex™ Ultra SP-L (containing pectinase, xylanase and cellulase activity) (1 ml) for complete precipitation of proteins at 4°C as described in the experimental section. The percentage activities of the enzymes in the precipitate were calculated by taking the initial activities as 100%. The experiments were carried out in triplicate and error bars represent the percentage error in each set of readings. -■-: pectinase, -□-: xylanase, --: cellulase.

Inline graphic — Precipitation of enzymatic activities with organic solvents: Three different precipitants (acetone, dimethoxyethane (DME) and n-propanol, 5 ml each) were added to crude Pectinex™ Ultra SP-L (containing pectinase, xylanase and cellulase activity) (1 ml) for complete precipitation of proteins at 4°C as described in the experimental section. The percentage activities of the enzymes in the precipitate were calculated by taking the initial activities as 100%. The experiments were carried out in triplicate and error bars represent the percentage error in each set of readings. -■-: pectinase, -□-: xylanase, --: cellulase.