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. 2007 Jun 8;1:16. doi: 10.1186/1752-153X-1-16

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Copyright © 2007 Dalal et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reusability of pectinase, xylanase and cellulase in CLEA: The reusability of pectinase in CLEA was studied by carrying out the hydrolysis of polygalacturonic acid at 30°C for 30 minutes. The reusability of xylanse and cellulase was studied by carrying out hydrolysis of xylan and carboxymethyl cellulose at 50°C for 30 minutes respectively. After each cycle, the reaction mixture was centrifuged at 10, 000 × g and the reducing sugar in the supernatant was measured using 3,5-dinitro salicylic acid. The CLEA recovered after centrifugation was used for next cycle of hydrolysis. The experiments were carried out in triplicate and error bars represent the percentage error in each set of readings.-■-: cellulase, -□-: xylanase, --: pectinase.

Inline graphic — Reusability of pectinase, xylanase and cellulase in CLEA: The reusability of pectinase in CLEA was studied by carrying out the hydrolysis of polygalacturonic acid at 30°C for 30 minutes. The reusability of xylanse and cellulase was studied by carrying out hydrolysis of xylan and carboxymethyl cellulose at 50°C for 30 minutes respectively. After each cycle, the reaction mixture was centrifuged at 10, 000 × g and the reducing sugar in the supernatant was measured using 3,5-dinitro salicylic acid. The CLEA recovered after centrifugation was used for next cycle of hydrolysis. The experiments were carried out in triplicate and error bars represent the percentage error in each set of readings.-■-: cellulase, -□-: xylanase, --: pectinase.