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Half-life of pectinase, xylanase and cellulase in CLEA
| Enzyme | Temperature (°C) | Half-life (t1/2) (minutes) | |
| Free | CLEAs | ||
| Pectinase | 50 | 17 | 180 |
| Xylanase | 60 | 22 | 82 |
| Cellulase | 70 | 32 | 91 |
The half-life values were calculated by plotting ln(residual activity of enzyme) vs time [19]. In all the three cases, the activity at 0 minute was taken as 100%. The experiments were carried out in triplicate and the percentage error in each set of readings was within 3%.
