Pre-acquisition scopolamine injections in the prelimbic cortex. (A) Latency to make the correct response (±SEM) over the three first acquisition trials (Acq123), the two last acquisition trials (Acq45), the two first 24-h test trials (Retention), and the two last 24-h test trials (Relearning). All groups show similar performance both in acquisition and 24-h test. (B) Number of errors before making the correct response (±SEM) over the three first acquisition trials (Acq123), the two last acquisition trials (Acq45), the two first 24-h test trials (Retention) and the two last 24-h test trials (Relearning). All groups show similar performance in the acquisition, but both SCOP groups commit more errors than the control group in the retention phase, and the SCOP20 group in the relearning the phase. (**) P ≤ 0.001, (*) P < 0.05.