Skip to main content
. Author manuscript; available in PMC: 2007 Sep 25.
Published in final edited form as: Biochemistry. 2006 Dec 26;45(51):15807–15816. doi: 10.1021/bi061803n

Figure 3. CYP2E1 and CYP2B4 mediated 7-pentoxyresorufin dealkylation (PROD) and aniline hydroxylation in simple and mixed reconstituted systems.

Figure 3

PROD and aniline hydroxylation was measured in simple reconstituted systems containing reductase and a single P450 (either CYP2E1 or CYP2B4), and mixed reconstituted systems containing reductase and both CYP2E1 and CYP2B4 in order to detect potential interactions between these P450 enzymes. Reconstitution and assay conditions are described in Methods and in the legend to Figure 1. Groups represent the mean ± SEM for three determinations. For both substrates, no significant difference between the sum of the activities of the binary systems (first two bars) and that of the mixed system (third bar).