Fig. 3.

Effects of vascular endothelial growth factor (VEGF) on medulloblastoma cell growth. Proliferation of DAOY, D283Med, and D341Med human medulloblastoma cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 72 h of VEGF₁₆₅ treatment (0, 10, 30, 50 ng/ml) with (squares) or without (triangles) the addition of VEGF inhibitor (80 μM). The maximal proliferative effect was seen using 30 ng/ml for DAOY cells (+67%) and D283Med (+9%). No significant differences in proliferation could be detected for the D341Med cell line. Proliferative response to VEGF was expressed as an increase in optical density (O.D.) with respect to the effect obtained with control. Values are means ± SD of triplicate experiments repeated twice. Student’s t-test: *p < 0.05; **p < 0.005. Abbreviation: ns, not significant.