Table 1.

Laboratory tests used to exclude other causes of amenorrhoea or hyperandrogenism.

Test	Exclusion	When to Perform
Free thyroxine (fT4) Thyroid stimulating hormone (TSH)	Thyroid disease	All Cases
Human Chorionic Gonadotrophin (hCG)	Pregnancy	If clinical suspicion or unexplained low LH/FSH
Prolactin	Hyperprolactinaemia	All cases
17- Hydroxyprogesterone Urinary steroid profile	Congenital adrenal hyperplasia	If total testosterone >4 nmol/L – see text and Table 4
Urinary free cortisol Evening serum or salivary cortisol	Cushing’s Syndrome	If clinical suspicion