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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Exp Eye Res. 2007 May 21;85(2):270–279. doi: 10.1016/j.exer.2007.05.001

Figure 1. Immunoprecipitation of TBP-2-TRx complex by anti-TRx and anti-TBP-2 antibodies.

Figure 1

Figure 1

Figure 1

HLE B3 cell lysate was incubated with either anti-TRx or anti-TBP-2 antibody. Protein-A Agarose was added and incubated as described in the Methods. Immunoprecipitate was then collected by centrifugation. A, SDS-PAGE analysis of immunoprecipitate obtained using anti-TRx antibody. Lane 1, Molecular weight marker; lane 2 Immunoprecipitate. B, SDS-PAGE of immunoprecipitate with anti-TBP-2 antibody. Lane 1, Molecular weight marker; lane 2 Immunoprecipitate. C, Western blot of the immunoprecipitate obtained with anti-TRx antibody. D, Western blot analysis of the immunoprecipitate obtained with anti-TBP-2 antibody. For western blot analysis immunoprecipitation was done using Seize® X Protein A Immunoprecipitation kit (PIERCE, IL).