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. Author manuscript; available in PMC: 2008 Aug 1.

Published in final edited form as: Exp Eye Res. 2007 May 21;85(2):270–279. doi: 10.1016/j.exer.2007.05.001

A, Western blot analysis of TBP-2 expression in control HLE B3 lysate (lane 1) and TBP-2 over-expressed HLE B3 lysate (lane 2). B, Comparison of the TRx activity in control and TBP-2 over-expressed HLE B3 cells. Cells from both groups (2 × 10⁶ cells from each group) were lysed and TRx activity determined as described. C, Comparison of growth rates of control (●) and TBP-2 over-expressed (■) cells during the 4-day growth period by cell counting. The results are based on the average of 3 determinations. Error bars indicate standard errors of the mean. D, Effect of TBP-2 over-expression on H₂O₂ mediated apoptosis. Control vector only cells and TBP-2 over-expressed cells (OE) were treated either with serum free MEM or with 100 μM H₂O₂ for 1 hr. Then the cells were washed with PBS and medium was replaced with 20% serum containing medium and incubated at 37°C for 16 hrs. Cells were trypsinized, washed with PBS, stained with Annexin V-FITC and analyzed by flow cytometry (for details see Materials and Methods). The results are based on the average of 3 determinations. Error bars indicate standard errors of the mean.

A, Western blot analysis of TBP-2 expression in control HLE B3 lysate (lane 1) and TBP-2 over-expressed HLE B3 lysate (lane 2). B, Comparison of the TRx activity in control and TBP-2 over-expressed HLE B3 cells. Cells from both groups (2 × 10⁶ cells from each group) were lysed and TRx activity determined as described. C, Comparison of growth rates of control (●) and TBP-2 over-expressed (■) cells during the 4-day growth period by cell counting. The results are based on the average of 3 determinations. Error bars indicate standard errors of the mean. D, Effect of TBP-2 over-expression on H₂O₂ mediated apoptosis. Control vector only cells and TBP-2 over-expressed cells (OE) were treated either with serum free MEM or with 100 μM H₂O₂ for 1 hr. Then the cells were washed with PBS and medium was replaced with 20% serum containing medium and incubated at 37°C for 16 hrs. Cells were trypsinized, washed with PBS, stained with Annexin V-FITC and analyzed by flow cytometry (for details see Materials and Methods). The results are based on the average of 3 determinations. Error bars indicate standard errors of the mean.