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. 2007 Aug 16;26(17):3910–3922. doi: 10.1038/sj.emboj.7601823

Figure 2.

Figure 2

Generation of TAX1BP1 knockout mice by gene trapping. (A) Genomic organization of the TAX1BP1 locus. PCR primers for genotyping are indicated by arrows. (B) PCR genotyping strategy for mice. The expected sizes of the PCR fragments are 275 bp with primers F1 and R2, and 154 bp with primers F1 and R1. RT–PCR using two sets of primers was performed with mRNA isolated from tails of four pups born from litters of a TAX1BP1 male chimera bred with a female C57BL/6 mouse. (C) TAX1BP1m/m embryos exhibit hemorrhaging at E13.5. Photographs of E13.5 TAX1BP1m/m and TAX1BP1+/m embryos. (D) The expression of TAX1BP1 and SV40 large T antigen (Tag) in TAX1BP1+/m and TAX1BP1m/m MEFs was determined by RT–PCR. The same primers (F1, R1 and R2) used in panel B were used to genotype MEFs. Primers specific to Tag were used to amplify a 500 bp fragment from Tag. (E) Whole-cell lysates from TAX1BP1+/m and TAX1BP1m/m MEFs were subjected to immunoblotting with polyclonal αTAX1BP1.