Fig. 2.
Enhanced detection of GαqQL-induced IP3 production by the trapping of metabolites. Rat1 cells were plated at densities of 25,000 cells per milliliter and labeled metabolically for 72 h with myo-[3H]inositol as described in Methods. Twenty-four hours before harvest, cells were infected with control adenovirus (labeled Mock) or GαqQL adenovirus (labeled Gαq). Lithium treatment was performed by adding LiCl to the cells at a final concentration of 10 mM 1 h before harvest. Soluble IPs were extracted by HCl and resolved by HPLC. Peaks were normalized to the total inositol counts present in the samples.