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Coexpression of activated Gαq with four of the six classes of IP kinases stimulates production of IP8. GαqQL and IPMK were coexpressed with IPK1, IPK1 + IP6K1, or IPK1 + IP6K + VIP1. Soluble IPs were extracted from the cells by using HCl and resolved by HPLC. Note the changes in the scale of the x and y axes from previous figures.
