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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: Mol Cell Endocrinol. 2007 May 29;274(1-2):35–42. doi: 10.1016/j.mce.2007.05.013

Fig.6. Effect of LPS on the GHR promoter is mediated via both MyD88-dependent and -independent pathways.

Fig.6

HEK 293T cells were transfected with L2-promoter-luciferase reporter (pGL3-L2-2.0 kb), pRL-TK (internal control), wild type TLR4 (0.1 ng/well), and wild type MD2 (0.1 ng/well) plasmids with or without dominant negative MyD88 or dominant negative TRIF plasmids. 48 h after transfection, cells were stimulated with LPS (1 μg/ml) for 5-6 h and cells harvested and luciferase activity measured. Results are expressed as mean ±SEM; n= 4 p < 0.05 (Mann-Whitney U test) compared to the luciferase activity in the absence of LPS (vehicle).