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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2007 Jul;48(7):3184–3194. doi: 10.1167/iovs.06-1403

Figure 3.

Figure 3

TGFβ2 effect on the alkaline phosphatase activity of primary HTM cells. Confluent HTM-63 cells in passage 5 were treated with 1 ng/mL and 3 ng/mL TGFβ2 for 3 days in serum-free medium. Extracts from harvested cells were divided into aliquots and evaluated for endogenous ALP and genomic DNA content. ALP levels were normalized to cell number, represented by total genomic DNA (mean ± SEM). *P ≤ 0.0002 (1 ng/mL, n = 3). *P ≤ 0.001 (3 ng/mL, n = 3). TGFβ2 significantly induces ALP in a dose-dependent manner in the primary HTM cells.